Production and characteristic of biologically active preparations from cattle bone meal
The problem of an ageing population is relevant for the whole world and for Russia in particular. The object of the research is cattle bone meal (CBM) proteoglycans. The methodology of the work included preparing a bone meal extract, a cluster silver solution, separating proteoglycans by electrophoresis, determining the uronic acids content in the investigated preparations by the carbazole method, defining the total polyanions content, chondroitinase activity. Then, high performance liquid chromatography for fractionating proteoglycans and MMT analysis were performed. It was determined that the bone meal extract preparation has biological activity. The investigated preparations, comparable in protein content, were tested for their ability to influence the survival of lymphocytes, healthy human B-cells. The preparation under study — CBM extract purified by using cluster silver — showed sensitivity to chondroitinase A, B, C, comparable to Theraflex and chemically homogeneous chondroitin, which gives ground to conclusion about the presence of chondroitin sulfates A, B, and C in the investigated preparation. The process of isolating biologically active components from CBM is described, which is characterized as chondroitin and heparan sulfates. The research results allowed obtaining biological preparations from meat production wastes that have biological activity and can be positioned as chondroprotectors similar to commercial preparations. In addition, the studies on cell culture have shown that extracts can replace animal blood serum, which is the most expensive cell culture reagent. The development and production of such preparations as the described CBM extract for scientific research would allow in the future to replace an expensive reagent with a much more economical domestic analogue, which has the prospect of being in demand.
Production and characteristic of biologically active preparations from cattle bone meal
The problem of an ageing population is relevant for the whole world and for Russia in particular. The object of the research is cattle bone meal (CBM) proteoglycans. The methodology of the work included preparing a bone meal extract, a cluster silver solution, separating proteoglycans by electrophoresis, determining the uronic acids content in the investigated preparations by the carbazole method, defining the total polyanions content, chondroitinase activity. Then, high performance liquid chromatography for fractionating proteoglycans and MMT analysis were performed. It was determined that the bone meal extract preparation has biological activity. The investigated preparations, comparable in protein content, were tested for their ability to influence the survival of lymphocytes, healthy human B-cells. The preparation under study — CBM extract purified by using cluster silver — showed sensitivity to chondroitinase A, B, C, comparable to Theraflex and chemically homogeneous chondroitin, which gives ground to conclusion about the presence of chondroitin sulfates A, B, and C in the investigated preparation. The process of isolating biologically active components from CBM is described, which is characterized as chondroitin and heparan sulfates. The research results allowed obtaining biological preparations from meat production wastes that have biological activity and can be positioned as chondroprotectors similar to commercial preparations. In addition, the studies on cell culture have shown that extracts can replace animal blood serum, which is the most expensive cell culture reagent. The development and production of such preparations as the described CBM extract for scientific research would allow in the future to replace an expensive reagent with a much more economical domestic analogue, which has the prospect of being in demand.
