Influence of Spermatozoa Quality on Rate of Oocyte Fertilization under the in Vitro Conditions
Quality control and standardization of genetic material and preparations used in animal reproduction are high-priority tasks for successful livestock management. A large amount of frozen semen is imported, but the material does not always meet needed quality standards. The quality indicators of semen production are influenced by many different factors, which include bull’s feeding and keeping, hygiene of semen collection, composition of diluents, methods of freezing and defrosting, and a number of other factors. The aim of the research was to study the influence of the quality parameters of spermatozoa obtained from different breeding bulls on oocyte fertilization in vitro. The sperm qualitative indicators (sperm activity, number of live and dead sperm cells, acrosome integrity) as well as the effect of these indicators on the oocyte fertilization under the in vitro conditions were studied on the material obtained from three servicing bulls. Oocytes for the experiment were obtained from donor cows by US-guided (ovum-pick-up (OPU)) aspiration from the ovaries of live animals at a livestock enterprise in the Kemerovo Region. Such sperm qualitative indicators of the bull Matias-4593 as the sperm activity (42.0±2.10%) and the number of sperm cells with damaged acrosome (25.2 ± 1.8%) positively influenced the fertilization rate of oocytes under the in vitro conditions, where 50% of fertilization cases were successful.
Influence of Spermatozoa Quality on Rate of Oocyte Fertilization under the in Vitro Conditions
Quality control and standardization of genetic material and preparations used in animal reproduction are high-priority tasks for successful livestock management. A large amount of frozen semen is imported, but the material does not always meet needed quality standards. The quality indicators of semen production are influenced by many different factors, which include bull’s feeding and keeping, hygiene of semen collection, composition of diluents, methods of freezing and defrosting, and a number of other factors. The aim of the research was to study the influence of the quality parameters of spermatozoa obtained from different breeding bulls on oocyte fertilization in vitro. The sperm qualitative indicators (sperm activity, number of live and dead sperm cells, acrosome integrity) as well as the effect of these indicators on the oocyte fertilization under the in vitro conditions were studied on the material obtained from three servicing bulls. Oocytes for the experiment were obtained from donor cows by US-guided (ovum-pick-up (OPU)) aspiration from the ovaries of live animals at a livestock enterprise in the Kemerovo Region. Such sperm qualitative indicators of the bull Matias-4593 as the sperm activity (42.0±2.10%) and the number of sperm cells with damaged acrosome (25.2 ± 1.8%) positively influenced the fertilization rate of oocytes under the in vitro conditions, where 50% of fertilization cases were successful.
Prevention of embryonic mortality in recipient cows
The results of research on the prevention of fetal mortality in recipient cows using a combination of anesthetic and sedative agents to relieve the stress response conducting embryo transplantation are presented. It is noted that during sacral anesthesia with 2% novocaine solution and intramuscular administration of 0.5 ml/ 100 kg of xylanite solution before embryo transplantation, the physiological parameters were within the limits of the physiological norm. Temperature indicators range from 38.4±0.42 to 39.0 ±0.14 ° C, pulse and respiration an hour after administration of drugs in the first experimental group corresponded to the physiological norm (67.6±0.83 beats per minute and 26.0±2.38 respiratory movements per minute). The dose of novocaine solution is calculated according to the instructions for the use of the drug, namely, the length of the croup is measured in cm (from the mucus to the sciatic tubercles) and divided by three, the resulting number denote the amount in ml of novocaine solution require for anesthesia.The level of cortisol is lower in the group with the use of a combination of drugs by 24.6% (P<0.01) than in the control one hour after the introduction of embryos into the female genital tract.
Prevention of embryonic mortality in recipient cows
The results of research on the prevention of fetal mortality in recipient cows using a combination of anesthetic and sedative agents to relieve the stress response conducting embryo transplantation are presented. It is noted that during sacral anesthesia with 2% novocaine solution and intramuscular administration of 0.5 ml/ 100 kg of xylanite solution before embryo transplantation, the physiological parameters were within the limits of the physiological norm. Temperature indicators range from 38.4±0.42 to 39.0 ±0.14 ° C, pulse and respiration an hour after administration of drugs in the first experimental group corresponded to the physiological norm (67.6±0.83 beats per minute and 26.0±2.38 respiratory movements per minute). The dose of novocaine solution is calculated according to the instructions for the use of the drug, namely, the length of the croup is measured in cm (from the mucus to the sciatic tubercles) and divided by three, the resulting number denote the amount in ml of novocaine solution require for anesthesia.The level of cortisol is lower in the group with the use of a combination of drugs by 24.6% (P<0.01) than in the control one hour after the introduction of embryos into the female genital tract.
Biochemical blood status in the intact pregnant cows and in those infected with the bovine leukemia virus
The aim of the research is to determine the differences of biochemical blood parameters in the intact cows and in those infected with the bovine leukemia virus (BLV). The feeding and keeping conditions for the livestock of both the control and experimental groups were similar, provided by the technology of the farm. Studying the blood samples was performed using traditional laboratory diagnostics methods. The results of the study were processed using biometric methods with determining the level of confidence based on the Student’s criterion. Significant differences were found between intact and infected animals in a number of indicators. The differences revealed in the biochemical parameters of the blood in BLV-infected and intact cows may indicate the development of pathological processes in the animals infected with the bovine leukemia virus.
Biochemical blood status in the intact pregnant cows and in those infected with the bovine leukemia virus
The aim of the research is to determine the differences of biochemical blood parameters in the intact cows and in those infected with the bovine leukemia virus (BLV). The feeding and keeping conditions for the livestock of both the control and experimental groups were similar, provided by the technology of the farm. Studying the blood samples was performed using traditional laboratory diagnostics methods. The results of the study were processed using biometric methods with determining the level of confidence based on the Student’s criterion. Significant differences were found between intact and infected animals in a number of indicators. The differences revealed in the biochemical parameters of the blood in BLV-infected and intact cows may indicate the development of pathological processes in the animals infected with the bovine leukemia virus.
