The present research has shown the growth and development of Nile tilapia (Oreochromis niloticus L.) fry effected from feeding Enzimsporin probiotic supplement. During the observation period, the weight of tilapia fry population has increased from 119.5g to 619g in the control group, from 121g to 684g in the 1st experimental group 1 and from 119g to 790g in the 2nd experimental group. Thus, the average daily weight gain per fish is 0.44g, 0.51g and 0.6g, respectively in 22 days.